Application

Research Sub CategoryIntegrins

Research CategoryCell Structure

Anti-Integrin β1 Antibody, clone HB1.1 detects level of Integrin β1 & has been published & validated for use in FC, IP, WB, IH.

Immunoprecipitation of VLA integrins from lysate of 10E6 cell equivalent requires 2 µg of MAB2000.

Flow cytometry: 10 µg/mL

Western blot

Immunohistochemistry in frozen tissue samples: 5 µg/mL

Induction of cell aggregation: 10 µg/mL. MAB2000 is a potent stimulator of cell aggregation tested in U937, Jurkat and human peripheral blood lymphocytes.

MAB2000 does not affect VLA integrin-mediated cell adhesion to matrix proteins.

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1109

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl with 0.1% sodium azide.

Specificity

Reacts with beta-1 subunit of VLA integrins

Storage and Stability

Maintain at 2-8°C for up to 6 months after date of receipt.